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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
22/11/2019 |
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Data da última atualização: |
10/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VIANA, C.; SERENO, M. J.; BERSOT, L. dos S.; KICH, J. D.; NERO, L. A. |
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Afiliação: |
CIBELI VIANA, UFV; MALLU JAGNOW SERENO, UFPR; LUCIANO DOS SANTOS BERSOT, UFPR; JALUSA DEON KICH, CNPSA; LUIS AUGUSTO NERO, UFV. |
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Título: |
Comparison of meat juice serology and bacteriology for surveillance of salmonella in the Brazilian pork production chain. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Foodborne Pathogens and Disease, v. 17, n. 3, p. 194-201, 2020. |
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DOI: |
10.1089/fpd.2019.2712 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the exception of results obtained by ELISA 40% cut-off from blood serum and meat juice with MLNs (K = 0.49 and 0.50, respectively) and tonsils (K = 0.29 and 0.30, respectively). Based on the obtained results, meat juice can be considered an alternative to blood serum as a matrix for ELISA for preliminary detection of Salmonella, allowing the identification of potential sources of contamination during slaughtering. MenosAbstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the except... Mostrar Tudo |
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Thesagro: |
Bacteriologia; Cadeia Produtiva; Carne; Elisa; Enzima; Salmonella; Suíno; Técnica Imunoenzimática. |
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Thesaurus NAL: |
Bacteriology; Disease surveillance; Enzyme-linked immunosorbent assay; Isolation techniques; Pork. |
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Categoria do assunto: |
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Marc: |
LEADER 02918naa a2200337 a 4500
001 2114901
005 2020-03-10
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1089/fpd.2019.2712$2DOI
100 1 $aVIANA, C.
245 $aComparison of meat juice serology and bacteriology for surveillance of salmonella in the Brazilian pork production chain.$h[electronic resource]
260 $c2020
520 $aAbstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the exception of results obtained by ELISA 40% cut-off from blood serum and meat juice with MLNs (K = 0.49 and 0.50, respectively) and tonsils (K = 0.29 and 0.30, respectively). Based on the obtained results, meat juice can be considered an alternative to blood serum as a matrix for ELISA for preliminary detection of Salmonella, allowing the identification of potential sources of contamination during slaughtering.
650 $aBacteriology
650 $aDisease surveillance
650 $aEnzyme-linked immunosorbent assay
650 $aIsolation techniques
650 $aPork
650 $aBacteriologia
650 $aCadeia Produtiva
650 $aCarne
650 $aElisa
650 $aEnzima
650 $aSalmonella
650 $aSuíno
650 $aTécnica Imunoenzimática
700 1 $aSERENO, M. J.
700 1 $aBERSOT, L. dos S.
700 1 $aKICH, J. D.
700 1 $aNERO, L. A.
773 $tFoodborne Pathogens and Disease$gv. 17, n. 3, p. 194-201, 2020.
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